A nurse needs to collect a urine specimen for culture and sensitivity from an adult client who has a long-term indwelling urinary catheter. Which action is appropriate to obtain the most accurate specimen?
Empty urine from the drainage bag into a sterile graduate and then pour it into the specimen container.
Disconnect the catheter from the drainage tubing and collect urine directly from the catheter into a sterile cup.
Clamp the catheter tubing below the sampling port, disinfect the port, and withdraw urine into a sterile syringe for transfer to a sterile container.
Attach a new sterile drainage bag and collect the first urine that drains into it for the specimen.
The nurse should clamp the catheter below the sampling port, cleanse the port with an antiseptic swab, and withdraw a fresh urine sample through the port using a sterile syringe before transferring it to a sterile container. Urine from the drainage bag or from disconnecting the catheter is likely to be contaminated or stale and can lead to inaccurate culture results. Collecting urine after simply replacing the drainage bag also allows old urine to mix with new urine, again risking contamination. Proper aseptic technique at the sampling port preserves specimen integrity and supports accurate diagnosis.
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NCLEX RN
Physiological Integrity
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