Trivial File Transfer Protocol (TFTP) uses UDP port 69 for its communication. This is the standard port designated for TFTP and it is important for network professionals to be aware of it for proper configuration and network traffic analysis. Other port numbers listed are not associated with TFTP and commonly refer to other services: port 22 is used by SSH and SFTP, port 25 is used by SMTP, and port 80 is used by HTTP.